南山茶果皮化学成分的研究

反复采用硅胶柱色谱法、Sephadex LH-20柱色谱法、ODS柱色谱法、反复重结晶等方法对南山茶果皮的化学成分进行分离纯化,并通过理化常数测定和波谱分析等方法进行结构鉴定。从南山茶果皮中分离并得到了10个化合物,分别鉴定为槲皮素(1)、山奈酚(2)、原儿茶酸(3)、山柰酚-3-O-β-D-葡萄糖基-(6→1)-2′′′,4′′′-O-二乙酰基-α-L-鼠李糖基-(3→1)-2′′′′,3′′′′,4′′′′-O-三乙酰基-α-L-鼠李糖苷(4)、山柰酚-3-O-β-D-葡萄糖基-(6→1)-4′′′-O-乙酰基-α-L-鼠李糖基-(3→1)-3′′′′-O-乙酰基-α-L-鼠李糖苷(5)、3-甲氧基鞣花酸(6)、鞣花酸(7)、羽扇豆烷醇(8)、β-谷甾醇(9)和胡萝卜苷(10)。化合物2、6、7均为首次从南山茶中分离得到,其中化合物6、7为首次从山茶属植物中分离得到。

Research on the chemical constituents of the fruit peels of Camellia semiserrata

The compounds were isolated from the peels of Camellia semiserrataby chromatography on silica gel column , Sephadex LH-20column , ODS column and recrystallization , and the structures were determined on the basis of physicochemical evidence and ectroscopic analysis.Ten compounds were isolated and identified as quercetin ( 1 ), kaempferol ( 2 ), protocatechuicacid ( 3 ), kaempferol-3-O- [( 2′′′′ , 3′′′′ , 4′′′′-triacetyl ) -α-L-rhamnopy-ranosyl ( 1→3 ) ( 2′′′ , 4′′′-diacetyl ) -α-L-rhamnopyranosyl ( 1→6 ) - β -D-glucopyranoside ]( 4 ), kaempferol-3-O- [( 3′′′′-acetyl ) -α-L- rhamnopyranosyl ( 1→3 )( 4′′′-acetyl ) -α-L-rhamnopyranosyl ( 1→6 ) - β -D-glucopyranoside ]( 5 ), 3′-O-methylellagic acid ( 6 ), ellagic acid ( 7 ), lupeol ( 8 ), β -sitosterol ( 9 ), daucosterol ( 10 ) .Compounds 2 , 6 and 7 were all isolated fromC. semiserratafor the first time.Compounds 6 and 7 were isolated from Camelliaspecies for the first time.