Isolation and characterization of human heart cytochromec oxidase |
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| Authors: | Lucia Kuhn-Nentwig Bernhard Kadenbach |
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| Affiliation: | (1) Biochemie, Fb Chemie der Philipps-Universität, Hans-Meerwein-Strasse, D-3550 Marburg, FRG |
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| Abstract: | Cytochromec oxidase was isolated from human hearts and separated by SDS gel electrophoresis. The identity of polypeptide bands with known subunits was demonstrated by immunoblotting with monospecific antisera to rat liver cytochromec oxidase subunits. The polarographically determined kinetics of cytochromec oxidation were similar to those reported for the bovine heart enzyme. |
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| Keywords: | Cytochromec oxidase human heart subunit composition monospecific antisera immunoblotting |
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