Sequence-ready physical map of the mouse Chromosome 16 region with conserved synteny to the human Velocardiofacial syndrome region on 22q11.2 |
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Authors: | James Lund Bruce Roe Feng Chen Marcia Budarf Naomi Galili Roy Riblet Robert D. Miller Beverly S. Emanuel Roger H. Reeves |
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Affiliation: | (1) Physiology 205, Johns Hopkins University School of Medicine, 725 N. Wolfe St., Baltimore, Maryland 21205, USA, US;(2) Dept. of Chemistry and Biochemistry, University of Oklahoma, Norman, Oklahoma 73019, USA, US;(3) Division of Human Genetics and Molecular Biology, The Children's Hospital of Philadelphia, Pennsylvania 19104, USA, US;(4) The Wistar Institute, Philadelphia, Pennsylvania 19104, USA, US;(5) Torrey Pines Institute for Molecular Studies, San Diego, California 92121, USA, US;(6) Dept. of Biology, University of New Mexico, Albuquerque, New Mexico, USA, US |
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Abstract: | Proximal mouse Chromosome (Chr) 16 shows conserved synteny with human Chrs 16, 8, 22, and 3. The mouse Chr 16/human Chr 22 conserved synteny region includes the DiGeorge/Velocardiofacial syndrome region of human Chr 22q11.2. A physical map of the entire mouse Chr 16/human Chr 22 region of conserved synteny has been constructed to provide a substrate for gene discovery, genomic sequencing, and animal model development. A YAC contig was constructed that extends ca. 5.4 Mb from a region of conserved synteny with human Chr 8 at Prkdc through the region conserved with human Chr 3 at DVL3. Sixty-one markers including 37 genes are mapped with average marker spacing of 90 kb. Physical distance was determined across the 2.6-Mb region from D16Mit74 to Hira with YAC fragmentation. The central region from D16Jhu28 to Igl-C1 was converted into BAC and PAC clones, further refining the physical map and providing sequence-ready template. The gene content and borders of three blocks of conserved linkage between human Chr 22q11.2 mouse Chr 16 are refined. Received: 4 November 1998 / Accepted: 21 December 1998 |
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