Proline reductase: a sensitive fluorometric assay with O-phthalaldehyde. |
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| Authors: | B Seto |
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| Affiliation: | Laboratory of Biochemistry, National Heart, Lung and Blood Institute, National Institutes of Health, Bethesda, Maryland 20014 USA |
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| Abstract: | A rapid and sensitive fluorometric assay for measuring the activity of proline reductase is described. The product of the enzymic reaction, δ-aminovalerate, is converted to a highly fluorescent derivative by reaction with o-phthalaldehyde. The water-soluble fluorescent product exhibits an excitation maximum at 340 nm and an emission maximum at 455 nm. The fluorophor reacts specifically with δ-aminovalerate without any interference from proline. |
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