Analysis of a novel antiinflammatory agent, 1-(7-tert.-butyl-2,3-dihydro-3,3-dimethylbenzo[b]furan-5-yl)-4-cyclopropylbutan-1-one (PGV-20229), in plasma matrices by stable-isotope-dilution gas chromatography-mass spectrometry |
| |
Authors: | Paula M Knight Michael W Scherz C A Cruze and Kenneth R Wehmeyer |
| |
Institution: | Procter & Gamble Phamaceuticals, Health Care Research Center, 8700 Mason-Montgomery Road, Mason, OH 45040, USA |
| |
Abstract: | A sensitive and selective GC-MS method was developed for the determination of low levels of a novel antiinflammatory agent, 1-(7-tert.-butyl-2,3-dihydro-3,3-dimethylbenzob]furan-5-yl)-4-cyclopropylbutan-1-one (I), in small volumes of animal plasma. The method involved the addition of 13C6-labeled-I to plasma samples, followed by a simple liquid-liquid extraction with hexane to isolate the analytes from matrix components. The levels of I in the sample extracts were determined by isotope-dilution GC-MS analysis using selected-ion monitoring. The method was linear over three orders of magnitude, with a limit of quantitation of 1.8 ng/ml I, using plasma sample volumes of 0.1 ml. The method was utilized to determine the pharmacokinetic parameters of I in rats and dogs, following intravenous administration. |
| |
Keywords: | Stable-isotope dilution PGV-20229 1-(7-tert -Butyl-2 3-dihydro-3 3-dimethylbenzo[b]furan-5-yl)-4-cyclopropylbutan-1-one |
本文献已被 ScienceDirect 等数据库收录! |
|