A linkage group on pig chromosome 4 comprising the loci for blood group L, GBA, ATP1B1 and three microsatellites |
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Authors: | L. Marklund,M. Johansson,U. Gustafsson,L. Andersson,A. K. Winterö ,M. Fredholm,P. D. Thomsen |
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Affiliation: | Department of Animal Breeding and Genetics, Swedish University of Agricultural Sciences, Box 7055, S-750 07 Uppsala, Sweden;Division of Animal Genetics, Department of Animal Science and Animal Health, The Royal Veterinary and Agricultural University, Frederiksberg C, Denmark;Division of Anatomy, Department of Anatomy and Physiology, The Royal Veterinary and Agricultural University, Fredriksberg C, Denmark |
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Abstract: | Restriction fragment length polymorphisms (RFLPs) were described for the porcine loci for β-glucosidase (GBA) and the β-polypeptide 1 of the Na+, K+-transporting ATPase (ATP1B1). Linkage analyses using a three-generation pedigree provided evidence for the assignment of ATP1B1, GBA and two microsatellite loci (S0001 and S0067) to a previously described linkage group comprising the loci for blood group L (EAL) and an anonymous microsatellite (S0097). The linear order of the six markers was determined with confidence by multipoint analyses and the length of the linkage group was estimated at 88 CM. This linkage group was assigned to pig chromosome 4 on the basis of a previous physical localization of the ATP1B1 gene. In situ hybridization data for S0001 presented in this study were consistent with a localization on chromosome 4 and suggested a regional localization to 4pl2-pl3. The present study reveals conflicting data concerning the genetic localization of the K88 loci controlling the expression of the receptors for the E. coli pilus antigens. One group has reported data suggesting a loose linkage between K88 and EAL, now mapped to chromosome 4, whereas two other groups have found linkage between K88 and the transferrin locus (TF), mapped to chromosome 13 by in situ hybridization. |
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Keywords: | pig gene mapping RFLP in situ hybridization blood group L |
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