沙门氏菌泛耐药基因组特征分析

[目的]分析沙门氏菌的泛耐药基因组特征.[方法]以EnteroBase数据库中16365株沙门氏菌为对象,利用课题组自主研发的泛耐药基因组分析软件(PRAP),进行泛耐药基因组结构的鉴定,通过曼-惠特尼秩和检验和皮尔逊检验,来分析耐药基因与血清型、序列型(sequence type,ST)及分离株样本来源信息间的相关性...

Characteristics analysis of pan-resistant genome of Salmonella

[Objective] Analyze the characteristics of the pan-resistant genome of Salmonella. [Methods] In this study, the pan-resistome analysis pipeline developed in our previous researches was used to identify the pan-resistant genomic structure and to analyze the correlation between drug-resistant genes and serotype, sequence type and isolation information, etc. based on the sequencing of 16,365 Salmonella strains downloaded from the EnteroBase database.[Results] All drug-resistant genes were categorized into 104 alleles, among which 18 alleles belonged to the core resistome and 86 alleles belonged to the accessory resistome. It had an open pan-resistant genome. The same serotype (or sequence type) had similar drug-resistant genes profile, however, the distribution of drug-resistant genes in different serotypes (or sequence types) is significantly different (P<0.05). Moreover, there was a certain correlation between drug-resistant genes and sample sources, separation countries and isolated years. The proportion of 23 acquired drug-resistant genes and their subtypes revealed that 10 (43.48%) of them increased yearly, and 10 (73.91%) of them had only one dominant type. [Conclusion] All the results obtained in this study have revealed the distribution and trend of Salmonella drug-resistant genes in recent years, in further to provide new ideas to study the drug-resistance of pathogens.