| CRISPR-Cas9技术在细菌耐药性防控研究进展 |
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| 引用本文： | 李培思,万鹏,李小申,崔世云,曾振灵.CRISPR-Cas9技术在细菌耐药性防控研究进展[J].微生物学报,2021,61(8):2161-2171. |
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| 作者姓名： | 李培思 万鹏 李小申 崔世云 曾振灵 |
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| 作者单位： | 华南农业大学/广东省兽药研制与安全评价重点实验室, 广东 广州 510642;岭南现代农业科学与技术广东省实验室, 广东 广州 510642;国家兽医微生物耐药性风险评估实验室, 广东 广州 510642 |
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| 基金项目： | 国家自然科学基金（31872524） |
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| 摘 要： | 近年来,多种新型耐药基因的出现和全球性流行,严重威胁了全球公众健康.CRISPR-Cas9系统(clustered regularly interspaced short palindromic repeats-CRISPR associated protein 9 system)是细菌的一种适应性免疫系统,可切割耐药...
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| 关 键 词： | CRISPR-Cas9系统 细菌耐药性 基因定向编辑 细菌基因组 质粒 |
| 收稿时间： | 2020/8/13 0:00:00 |
| 修稿时间： | 2020/9/14 0:00:00 |
CRISPR-Cas9 technology in the prevention and control of antibiotic resistance |
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| Peisi Li,Peng Wan,Xiaoshen Li,Shiyun Cui,Zhenling Zeng.CRISPR-Cas9 technology in the prevention and control of antibiotic resistance[J].Acta Microbiologica Sinica,2021,61(8):2161-2171. |
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| Authors: | Peisi Li Peng Wan Xiaoshen Li Shiyun Cui Zhenling Zeng |
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| Affiliation: | Guangdong Provincial Key Laboratory of Veterinary Pharmaceutics Development and Safety Evaluation, South China Agricultural University, Guangzhou 510642, Guangdong Province, China;Guangdong Laboratory for Lingnan Modern Agriculture, Guangzhou 510642, Guangdong Province, China;National Risk Assessment Laboratory for Antimicrobial Resistance of Animal Original Bacteria, Guangzhou 510642, Guangdong Province, China |
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| Abstract: | Recently, the emergence and global prevalence of many new antibiotic genes have led to a worldwide public health crisis. CRISPR-Cas9 (an adaptive immune system of bacteria) that can be used to cleave specific targets of resistant bacteria and against exogenous nucleic acid, serves a revolutionary gene editing tool to control antimicrobial resistance. Our previous study has established a plasmid-mediated CRISPR-Cas9 system targeting the mcr-1 gene, to efficiently and specifically eliminate mcr-1 gene and resensitize colistin resistant E. coli. Moreover, it is also necessary to optimize delivery methods of the CRISPR-Cas9 system for clinical treatment. This review focuses on the process of CRISPR-Cas9 technology in the prevention and control of antibiotic resistance in recent years, including the discovery process, mechanism, delivery method, advances in the experimental results in vitro and the existing problems, to provide new insights to the prevention and control of antibiotic resistance. |
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| Keywords: | CRISPR-Cas9 system antibiotic resistance gene targeted editing bacterial genome plasmid |
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