菌群失调腹泻造模及七味白术散治疗对小鼠乳糖酶基因13910多态性的影响

目的探讨抗生素及七味白术散对菌群失调腹泻小鼠乳糖酶基因13910位点多态性的影响。方法将36只SPF级小鼠随机分为正常组(12只)、模型组(12只)、七味白术散组(6只)和乳糖酶组(6只),除正常组外的其余小鼠采用混合抗生素造成菌群失调腹泻模型,然后分别灌胃七味白术散和乳糖酶治疗。造模成功和治疗后分别提取小鼠肠道内容物和肠道黏膜宏基因组,对包括小鼠乳糖酶基因13910位点在内的1 036bp长度的DNA片段进行单核苷酸多态性(SNP)测序分析。结果造模和治疗后,小鼠乳糖酶基因13910位点没有发生碱基的变异,均为A/A型,13910位点在内的1 036bp范围内也没有新的SNP位点出现。结论菌群失调腹泻造模与七味白术散治疗对小鼠乳糖酶活性的干预均与小鼠乳糖酶基因13910位点多态性没有相关性,可能存在其他多态性位点或其他方面的调控机制。

Effects of modeling and Qiwei Baizhu San on polymorphism of lactase gene 13910 in mice with dysbacteriosis diarrhea

Abstract: Objective To study the effects of antibiotics and Qiwei Baizhu San on the polymorphism of lactase gene 13910 in mice with dysbacteriosis diarrhea. Methods 36 SPF mice were randomly divided into normal group (n=12), model group (n=12), Qiwei Baizhu San group (n=6) or lactase group (n=6). All mice except those in the normal group were gavaged with mixed antibiotics to establish the models of dysbacteriosis diarrhea, and then given treatment with Qiwei Baizhu San or lactase respectively after the modeling. The intestinal contents and intestinal mucosal metagenome of mice were respectively extracted after successful modeling and treatment, and the 1,036 bp DNA fragment including 13910 site was analyzed by using SNP sequencing. Results After modeling and treatment, the lactase gene 13910 site was found to be A/A type without any base mutations, and no new SNP site was detected in the 1,036 bp range including the 13910 site. Conclusion The intervention of lactase activity by dysbacteriosis diarrhea modeling with antibiotics and treatment with Qiwei Baizhu San have no correlation with the polymorphism of lactase gene in mice; there may be other SNP site or other regulatory mechanisms.