Fucoidan对巨噬细胞RAW264.7的免疫调节作用

目的 观察褐藻多糖硫酸酯（Fucoidan）对巨噬细胞RAW264.7体外吞噬活性、细胞因子TNF-α和IL-6分泌，以及Toll样受体4（TLR4）mRNA表达的影响。方法 实验分对照组，Fucoidan高、中、低剂量组（浓度分别是200、400和800 μg/mL）。药物处理6~48 h后，MTT法检测RAW264.7细胞活力；中性红比色法检测细胞吞噬活性；ELISA法检测培养上清中TNF-α和IL-6的分泌水平；实时定量PCR检测Toll样受体4（TLR4）mRNA表达量。结果 与对照组相比，Fucoidan显著增强RAW264.7细胞代谢活力和吞噬能力（P＜0.01），增加TNF-α和IL-6的分泌，上调TLR4的表达，呈剂量依赖关系。结论 Fucoidan可上调TLR4表达，增强巨噬细胞代谢和吞噬活性，增加TNF-α和IL-6的分泌，具有潜在的调节免疫作用。

Immunomodulatory effects of fucoidan from Undaria pinnatifida sporophyll on macrophage RAW264.7

Objective To observe the effects of fucoidan on phagocytic activity in vitro, secretion of cytokines TNF-α and IL-6, and the mRNA level of toll-like receptor 4 (TLR4) in mouse macrophage RAW264.7. Methods The cell viability and phagocytic activity of RAW264.7 treated with fucoidan (at concentrations of 0, 200, 400 and 800 μg/mL respectively for 6 to 48 hours) were detected by using MTT and neutral red colorimetry respectively in vitro. The effect of fucoidan on the levels of TNF-α and IL-6 in RAW264.7 cells was assessed with ELISA assay. The mRNA level of TLR4 was tested by using real-time quantitative PCR (RT-PCR). Results Compared with the control group, fucoidan significantly enhanced the vitality and phagocytosis of RAW264.7 cells (P<0.01), increased the secretion of TNF-α and IL-6 and the expression of TLR4 in a dose-dependent manner. Conclusion Fucoidan can significantly increase TLR4 expression, enhance the metabolism and phagocytosis of macrophage RAW264.7, and promote the secretion of TNF-α and IL-6, which has a potential application value in regulating immune imbalance.