一株高效降解纤维素菌株的筛选及其产酶能力研究

目的 筛选并鉴定一种产纤维素酶能力较高的菌株，为纤维素的高效利用贮备菌源。方法 用羧甲基纤维素钠（CMC-Na）平板筛选产纤维素酶菌株，通过LB培养基对其进行纯化，16S rDNA基因序列分析其分类地位，3,5-二硝基水杨酸法（DNS）测定其产酶能力。结果 分离纯化得到的产纤维素酶菌株（S1）为芽胞杆菌属（Bacillus genus）的短小芽胞菌，在最佳产酶条件下产酶含量达到1 204 U/mL，产纤维素酶能力与里氏木霉（Trichoderma reesei）相当，但其产酶速率较里氏木霉低。结论 S1是一株产纤维素酶能力较高的菌株，产酶条件温和，初步鉴定为一种新种，具有较高研究及应用价值。

Screening and enzymatic productivity of a strain of efficient cellulose hydrolyzing bacteria

Objective To isolate and identify highly efficient cellulose-producing strain for microbial resource. Methods CMC-Na medium was used to screen cellulose-degrading strains initially and LB medium was used to purify them. The identification of 16S rDNA was performed, and enzymatic productivity was determined by using DNS method. Results The strain S1 was identified as a strain of Bacillus genus, which was capable of producing cellulose with a productivity of 1,204 U/mL under the optimal culturing condition, similar to that of Trichoderma reesei, but its enzyme production rate was lower than that of Trichoderma reesei. Conclusion Strain S1 is able to produce cellulase efficiently under mild conditions, which as a novel species, has a high value of application and is worth of further research.