| 根癌农杆菌介导AtNHX1基因转化番茄的研究 |
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| 引用本文： | 朱莹,周琦,崔继哲. 根癌农杆菌介导AtNHX1基因转化番茄的研究[J]. 生物技术通报, 2010, 0(3) |
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| 作者姓名： | 朱莹 周琦 崔继哲 |
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| 作者单位： | 哈尔滨师范大学生命科学与技术学院,哈尔滨,150025 |
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| 基金项目： | 黑龙江省科技攻关计划项目 |
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| 摘 要： | 构建AtNHX1基因植物表达载体,通过农杆菌介导法将其转入番茄。探讨了外植体类型、农杆菌菌株和不同筛选标记对芽诱导分化的影响。对抗性植株进行PCR检测,获得15株转基因植株。对转基因番茄T1代进行80mmol/LNaHCO胁迫处理,转基因植株的相对生长量高于对照植株,显示AtNHX1基因的导入提高了番茄对碱性盐的耐受性。
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| 关 键 词： | 番茄 AtNHX1基因 农杆菌介导法 NaHCO_3胁迫 |
Agrobacterium-Mediated Transformation of AtNHX1 Gene into Tomato |
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| Zhu Ying,Zhou Qi,Cui Jizhe. Agrobacterium-Mediated Transformation of AtNHX1 Gene into Tomato[J]. Biotechnology Bulletin, 2010, 0(3) |
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| Authors: | Zhu Ying Zhou Qi Cui Jizhe |
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| Affiliation: | Zhu Ying Zhou Qi Cui Jizhe(College of Life Sciences , Biotechnology,Harbin Normal University,Harbin 150025) |
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| Abstract: | AtNHX1 gene was successfully transferred into tomato by Agrobacterium-mediated transformation.Several factors that affect the frequency of shoot differentiation such as types of explants,Agrobacterium strains,vectors with different selectable markers were studied.The resistant plants were confirmed by PCR assay,15 transgenic plants were obtained.Relative growth was estimated when they were cultured under 80 mmol/L NaHCO3 stress.The results indicated that the relative growth of the transgenic plants was high... |
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| Keywords: | Tomato AtNHX1 gene Agrobacterium-mediated method NaHCO_3 stress |
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