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| DNA-PKcs基因沉默抑制人乳腺上皮细胞对低剂量辐射损伤的修复 | |
| 引用本文： | 邹伟,车鉴,王崇杰,崔玉影,张钦明. DNA-PKcs基因沉默抑制人乳腺上皮细胞对低剂量辐射损伤的修复[J]. 生物工程学报, 2009, 25(5): 727-732 |
| 作者姓名： | 邹伟 车鉴 王崇杰 崔玉影 张钦明 |
| 作者单位： | 1. 辽宁师范大学生命科学学院,大连,116029;辽宁省生物技术和分子药物研发重点实验室,大连,116029;美国科罗拉多州立大学环境与放射卫生学系,柯林斯堡,80523 2. 辽宁师范大学生命科学学院,大连,116029 3. 辽宁师范大学物理学院,大连,116029 4. 美国科罗拉多州立大学环境与放射卫生学系,柯林斯堡,80523 |
| 基金项目： | 国家自然科学基金项目(No.30570225);;辽宁省教育厅科研基金资助项目(No.05L206)资助~~ |
| 摘 要： | DNA-PKcs作为DNA依赖性蛋白激酶(DNA-PK)的催化亚基在DNA双链断裂(DSBs)的非同源末端重组(NHEJ)通路中起重要的作用。本实验以人乳腺上皮细胞株MCF10F为研究对象,通过siRNA技术抑制细胞内DNA-PKcs的表达,用50cGy137CS照射细胞,测定细胞生长曲线以确定细胞对低剂量辐射(LDR)的敏感性,同时检测DNA修复相关蛋白表达的变化,旨在探讨DNA依赖蛋白激酶(DNA-PKcs)基因沉默对人乳腺上皮细胞株MCF10F低剂量辐射敏感性的影响及机制。结果显示:转染特异性siRNA可使人乳腺上皮细胞(MCF10F)DNA-PKcs基因沉默,增殖受到明显的抑制;50cGyγ射线辐射可使乳腺细胞内DNA-PKcs、Ku80、ATM、P53等DNA修复相关蛋白表达增多,但DNA-PKcs基因沉默细胞(MCF10Fpk)中,这些蛋白表达显著低于对照组(MCF10Fmock)。以上结果提示,DNA-PKcs基因沉默可引起乳腺细胞对低剂量辐射敏感性增加,其原因可能与相关DNA修复蛋白表达减少有关。 |
| 关 键 词： | 乳腺细胞 基因沉默 DNA-PKcs基因 |
| 收稿时间： | 2009-01-15 |
DNA-PKcs silencing inhibit the DNA repair induced by low dose radiation on human breast epithelial cells |
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| Wei Zou,Jian Che,Chongjie Wang,Yuying Cui and Qinming Zhang. DNA-PKcs silencing inhibit the DNA repair induced by low dose radiation on human breast epithelial cells[J]. Chinese journal of biotechnology, 2009, 25(5): 727-732 | |
| Authors: | Wei Zou Jian Che Chongjie Wang Yuying Cui Qinming Zhang |
| Affiliation: | College of Life Sciences, Liaoning Normal University, Dalian 116029, China; Liaoning Key Laboratories of Biotechnology and Molecular Drug Research and Development, Dalian 116029, China; Departments of Environmental and Radiological Health Sciences, Colora;College of Life Sciences, Liaoning Normal University, Dalian 116029, China;Institute of Physics, Liaoning Normal University, Dalian 116029, China;College of Life Sciences, Liaoning Normal University, Dalian 116029, China;Departments of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins 80523, USA |
| Abstract: | DNA-PKcs, the catalytic subunit of DNA-dependent protein kinase(DNA-PK), plays an important role in the nonhomologous end-joining(NHEJ) pathway of DNA double-strand breaks(DSBs) repair.To investigate the effects of DNA-PKcs down-regulation on cell growth and sensitization to low dose radiation(LDR), we transfected DNA-PKcs siRNA into human mammary epithelia cells MCF10F, then, detected the proliferation curve of the cells and the expression of protiens in DNA repair pathways.The results showed that DNA-PKcs... |
| Keywords: | breast epithelial cell line gene silence DNA-PKcs gene |
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