Effect of beta-xylosides on synthesis of cartilage-specific proteoglycan in chondrocyte cultures. |
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Authors: | N B Schwartz P L Ho A Dorfman |
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Institution: | 1. Department of Pediatrics Joseph P. Kennedy, Jr. Mental Retardation Research Center Pritzker School of Medicine University of Chicago Chicago, Illinois 60637 USA;2. Department of Biochemistry Joseph P. Kennedy, Jr. Mental Retardation Research Center Pritzker School of Medicine University of Chicago Chicago, Illinois 60637 USA |
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Abstract: | Monolayer cultures of embryonic chick chondrocytes were incubated with 35SO4 in the presence and absence of 1.0 mM p-nitrophenyl-β-D-xylose for 2 days. The relative amounts of chondroitin sulfate proteoglycan and free chondroitin sulfate chains were measured following gel filtration on Sephadex G-200. Synthesis of β-xyloside-initiated polysaccharide chains was accompanied by an apparent decrease in chondroitin sulfate proteoglycan production by the treated cultures. The amount of core protein was determined from equivalent numbers of β-xyloside-treated and untreated cells by a radioimmune assay. Similar amounts of core protein were found in both types of cultures, indicating that decreased synthesis of cartilage-specific core protein is not responsible for the observed decrease in overall chondroitin sulfate proteoglycan production. |
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