常用实验用近交系小鼠粒体ＤＮＡ遗传变异的分析

应用ＰＣＲ－ＲＦＬＰ和ＰＣＲ－ＳＳＣＰ技术，研究了分析了国内常用的实验用近交系小鼠线粒体ＤＮＡ（mtＤＮＡ）的品种间遗传变异。ＰＣＲ－ＲＦＬＰ分析发现，小鼠mtＤＮＡ的Ｄ－loop、tRNA^Met Glu Ile及ＮＤ３基因核酸序列，在４６个限制性内切酶酶切位点上均无差异；用ＰＣＲ－ＳＳＣＰ分析方法对这些小鼠mtＤＮＡ的高变异区Ｄ－Loop的５′及3′端作进一步分析，亦未发现品种间遗传变异。结合mtＤＮＡ具有的母系遗传方式的特点，这一结果提示：常用的实验用近交系小鼠形成中可能只有１种雌性血统起了作用。

The Study of Genetic Variability of mtDNA in Common Laboratory Inbred Strains of Mice

The genetic viriation of mtDNA among 7 common laboratory inbred strains of mice was analyzed by PCR-RFLP and PCR-SSCP techniques. D杔oop, tRNAMet＋Glu＋Ile and ND3 fragments of mtDNA from the mices showed no variation in 46 endonuclease sites; Deeply analyzed by PCR-SSCP, the D杔oop 5′ fagment and 3′ fragment of mtDNA from these mice also show no genetic viriation. Because of maternal mode of inheritance of mtDNA, the results indicate that only one female lineage contributed to the formation of all these common inbred strains of mice.