Effect of carbon dioxide on cell growth and saponin production in suspension cultures of Panax ginseng |
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Authors: | N. T. Thanh H. N. Murthy D. M. Pandey K. W. Yu E. J. Hahn K. Y. Paek |
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Affiliation: | (1) Research Centre for the Deelopment of Advanced Horticultural Technology, Chungbuk National University, Cheongju, 361763, South Korea;(2) Department of Botany, Karnatak University, Dharwad, 580003, India |
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Abstract: | The effects of carbon dioxide supply within the range of 1–5 % (along with purified air), on cell culture of Panax ginseng were investigated in a balloon type bubble bioreactor containing 4 dm3 of Murashige and Skoog (MS) medium supplemented with 7.0 mg dm−3 indolebutyric acid, 0.5 mg dm−3 kinetin and 30 g dm−3 sucrose. A 1 % CO2 supply was found beneficial for the production of cell mass; however, increasing CO2 concentration to 2.5 and 5 % decreased the biomass accumulation. CO2 enrichment was not beneficial for saponin production and 1, 2.5, and 5 % CO2 supply resulted in decrease in saponin accumulation up to 11.6, 19.5, and 50.6 %, respectively. |
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Keywords: | bioreactors ginseng plant cell culture secondary metabolites |
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