吸附无细胞百白破(DPTa)的稳定性试验 |
| |
引用本文: | 傅惠兰 钟泽荣. 吸附无细胞百白破(DPTa)的稳定性试验[J]. 微生物学免疫学进展, 1998, 26(1): 10-12 |
| |
作者姓名: | 傅惠兰 钟泽荣 |
| |
作者单位: | 卫生部成都生物制品研究所 |
| |
摘 要: | 采用半综合液体培养基(含0.05%MeβCD),大罐培养百日咳Ⅰ相CS菌制备的吸附无细胞百日咳菌苗、白喉、破伤风类毒素混合制剂(DPTa)置4-8℃分别保存一年、保存两年,测三种抗原成分的效力及毒性,以及将该制剂于37℃分别放置三周、三个月,测定有无毒性逆转。结果表明,该制剂质量稳定,无毒性逆转
|
关 键 词: | DPTa 效力 毒性 稳定性 |
The Stability Test of Absorbed Acellular Pertussis Vaccine Diphtheria and Tetanus Toxoid(DPTa) |
| |
Abstract: | This paper reports that pertussiscs phase Ⅰ was cultivated in a 500- liter fermentor using a semisynthetic medium containing 0.05% heptakis (dimethyl b cyclodextrin).The protective antigens were purified from the culture supernatant and mixed with diphtheria and tetanus toxoied and absorbed with alumiunum compound to form DPTa.This preparation was stored at 4-8℃ for one year and two years.The potency of three antigens and toxicity of acellular pertussis vaccine were tested.Sinultaneously the preparation was incubated at 37℃ both three weeks and three months.Toxicity reversion of acellular pertussis vaccine was tested too.The tests showed that potency and toxicity of DPTa stored in one year and two years at 4-8℃ were stable,and toxicity revergion of acellular pertussis vaccine incubated at 37℃ both three weeks and three months was not found. |
| |
Keywords: | DPTa Potency Toxicity Stability |
本文献已被 CNKI 维普 等数据库收录! |