吸附无细胞百白破(DPTa)的稳定性试验 |
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引用本文: | 傅惠兰,钟泽荣.吸附无细胞百白破(DPTa)的稳定性试验[J].微生物学免疫学进展,1998,26(1):10-12. |
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作者姓名: | 傅惠兰 钟泽荣 |
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作者单位: | 卫生部成都生物制品研究所 |
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摘 要: | 采用半综合液体培养基(含0.05%MeβCD),大罐培养百日咳Ⅰ相CS菌制备的吸附无细胞百日咳菌苗、白喉、破伤风类毒素混合制剂(DPTa)置4-8℃分别保存一年、保存两年,测三种抗原成分的效力及毒性,以及将该制剂于37℃分别放置三周、三个月,测定有无毒性逆转。结果表明,该制剂质量稳定,无毒性逆转
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关 键 词: | DPTa 效力 毒性 稳定性 |
The Stability Test of Absorbed Acellular Pertussis Vaccine Diphtheria and Tetanus Toxoid(DPTa) |
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Abstract: | This paper reports that pertussiscs phase Ⅰ was cultivated in a 500- liter fermentor using a semisynthetic medium containing 0.05% heptakis (dimethyl b cyclodextrin).The protective antigens were purified from the culture supernatant and mixed with diphtheria and tetanus toxoied and absorbed with alumiunum compound to form DPTa.This preparation was stored at 4-8℃ for one year and two years.The potency of three antigens and toxicity of acellular pertussis vaccine were tested.Sinultaneously the preparation was incubated at 37℃ both three weeks and three months.Toxicity reversion of acellular pertussis vaccine was tested too.The tests showed that potency and toxicity of DPTa stored in one year and two years at 4-8℃ were stable,and toxicity revergion of acellular pertussis vaccine incubated at 37℃ both three weeks and three months was not found. |
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Keywords: | DPTa Potency Toxicity Stability |
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