Dilatation of Golgi vesicles by monensin leads to enhanced accumulation of sugar nucleotides |
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Authors: | Roméo Cecchelli René Cacan Eliane Porchet-Hennere André Verbert |
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Affiliation: | (1) Laboratoire Associé au CNRS no. 148, Université des Sciences et Techniques de Lille, 59655 Villeneuve d'Ascq Cédex, France |
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Abstract: | Incubation of mouse thymocytes with 10M monensin for 1 hour induces morphological alterations characterized by the extensive dilatation and vacuolization of the Golgi complex. This effect is used to study the transport and utilization of labelled sugar nucleotides into intracellular vesicles by using thymocytes whose plasma membrane has been permeabilized by ammonium chloride treatment. It is demonstrated that monensin stimulates the incorporation of labelled sialyl, fucosyl, galactosyl, and N-acetylglucosaminyl residues. This enhanced incorporation is not due to a direct effect of monensin on glycosyltransferase activities themselves but is a consequence of a higher entry and accumulation of labelled sugar nucleotides in the dilated vesicles.Laboratoire de Chimie Biologique and Laboratoire Associé au CNRS no. 217. |
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Keywords: | Sugar nucleotide transport monensin glycosylation cell permeabilisation |
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