Purification and properties of a beta-galactoside-binding lectin from neonatal marmoset. |
| |
Authors: | F Ohsawa F Hirano S Natori |
| |
Affiliation: | Pharmaceutical Research Laboratories, Meiji Seika Kaisha, Ltd., Kanagawa. |
| |
Abstract: | A beta-galactoside-binding lectin was extracted from whole neonatal marmoset homogenate with lactose solution and purified to homogeneity by ion-exchange chromatography on Q Sepharose Fast Flow and by affinity adsorption to trypsinized and glutaraldehyde-fixed ghosts of rabbit erythrocytes. The lectin has a dimeric structure composed of two 15K subunits. Its amino acid composition and partial amino acid sequences were quite similar to those of beta-galactoside-binding lectins from human placenta and lung. |
| |
Keywords: | |
|
|