An improved method for staining cells of the endocrine polypeptide (APUD) series by masked metachromasia: application of the principle of 'fixation by excluded volume'. |
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| Authors: | M. Cecilia M. Rost F. W. D. Rost |
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| Affiliation: | (1) Department of Histochemistry, Royal Postgraduate Medical School, London, UK;(2) Present address: Department of Pathology, University of Sydney, Sydney, Australia;(3) Present address: School of Anatomy, University of New South Wales, Sydney, Australia |
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| Abstract: | Synopsis Masked metachromasia is demonstrated by staining with a metachromatic basic dye, after acid hydrolysis of suitably fixed tissue. We report that the addition of 20% Carbowax 20M (an inert polymer, mol. wt. about 20000) to the hydrolysis mixture improved the reaction. The improved method gives increased metachromasia, greater tolerance to variations in hydrolysis conditions, and demonstrates a greater proportion of cells — presumably due to a lower threshold of sensitivity. Lower molecular weight polymers (Carbowax 1000, Carbowax 6000) are less effective. |
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