Production of Linolenic Acid by Mortierella isabellina Grown on Octadecanol |
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| Authors: | Mo Xian Yijian Kang Jicang Yan Junhong Liu Yingli Bi Kaiji Zhen |
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| Institution: | (1) Department of Chemistry, Jilin University, Changchun 130023, China, CN;(2) Department of Applied Chemistry, Qingdao Institute of Chemical Technology, Qingdao 266042, China, CN;(3) Department of Chemistry, Northeast Normal University, Changchun 130024, China, CN |
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| Abstract: | The production of linolenic acid in mycelial lipids reached 0.31 mg/ml of culture broth when Mortierella isabellina was cultivated in a medium consisting of 2% octadecanol, 1% yeast extract, and 25 mmol/L of Mg2+ at 23°C for 5 days. Cultivation conditions were studied, and the results showed that (i) a suitable concentration of Mg2+ in the medium caused an increase in mycelial mass as well as linolenic acid production; (ii) when incubated at 23°C, maximal
linolenic acid productivity was reached, although a higher content of the acid in total fatty acids was found at the lower
temperature; (iii) the effect of substrate concentration on linolenic acid yield showed that the latter increased with concentration
of substrate, and maximal linolenic acid yield was obtained with concentrations of 2% octadecanol and 1% yeast extract.
Received: 27 November 2000 / Accepted: 22 June 2001 |
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