黔产剑叶凤尾蕨的化学成分研究

为研究剑叶凤尾蕨(Pteris ensiformis)的化学成分，该研究选用硅胶、凝胶、MCI、C₁₈等柱色谱进行分离纯化，结合¹H-NMR、¹³C-NMR、MS、IR等波谱数据鉴定化合物结构，并通过MTS和APTT、PT以及TT等方法对所分离得到的部分单体化合物进行抗肿瘤和抗凝血活性筛选。结果表明：(1)从剑叶凤尾蕨中分离得到15个化合物，分别为2-羟基-乙酰基吡咯(1)、N-(3-羧丙基)-2-乙酰基吡咯(2)、3-羟基-2-甲基吡啶(3)、N-甲基羟胺(4)、pterosin S 13-O-glucoside(5)、obtupterosin C(6)、ent-11α-hydroxy-15-oxokauran-19-oic acid(7)、ent-11α-hydroxy-15-oxokaur-16-en-19-oic acid(8)、β-谷甾醇(9)、ent-11α-hydroxy-15-oxokaur-16-en-19-oic acid-O-glucopyranoside(10)、5, 5′-二丁氧基-2, 2′-...

Chemical constituents of Pteris ensiformis from Guizhou

To study the chemical constituents of Pteris ensiformis, silica gel, gel, MCI, C₁₈ and other column chromatography were used for separation and purification, and their structures were identified by ¹H-NMR, ¹³C-NMR, MS, IR and othe spectral data; the anti-tumor and anti-coagulation activities of some monomers were screened by MTS, APTT, PT and TT. The results were as follows:(1)A total of 15 compounds were isolated from P. ensiformis, the compounds were 2-hydroxy-acetylpyrrole(1), N-(3-carboxypropyl)-2-acetylpyrrole(2), 3-hydroxy-2-methylpyridine(3), N-methylhydroxylamine(4), pterosin S 13-O-glucoside(5), obtupterosin C(6), ent-11α-hydroxy-15-oxokauran-19-oic acid(7), ent-11α-hydroxy-15-oxokaur-16-en-19-oic acid(8), β-sitosterol(9), ent-11α-hydroxy-15-oxokaur-16-en-19-oic acid-O-glucopyranoside(10), 5, 5''-dibutoxy-2, 2''-bifuran(11), 5, 5''-di(2-ethyl-hexyloxy)-2, 2''-bifuran(12),(-)-loliolide(13), succinic acid(14), fumaric acid(15). Compound 1 is a new natural product of pyrrole alkaloids. Compounds 1-7, 10-15 were isolated from P. ensiformis for the first time, and compounds 1, 3, 4 were isolated from Pteris for the first time.(2)The results of activity test showed that compounds 1, 2, 3, 5, 6 and 10 inhibited the growth of tumor cells HL-60, A549, SMMC-7721, MDA-MB-231 and SW480 in vitro at a concentration of 40 μmol·L^-1, At the concentration of 2.0 mmol·L^-1, compounds 1, 2, 3 and 6 shortened APTT and compounds 1, 5 and 6 prolonged PT. The study enriches the chemical constituents of P. ensiformis from Guizhou and provides a material basis for the development of anti-tumor drugs.