|
|||||
|
|
PhiC31 integrase induces a DNA damage response and chromosomal rearrangements in human adult fibroblasts |
|
| Authors: | Jian Liu Tina Skj?rringe Torben Gjetting Thomas G Jensen |
| Affiliation: | (1) The Kennedy Center, Gl. Landevej 7, 2600 Glostrup, Denmark;(2) Department of Laboratory Medicine, Clinical Research Center, Malm? University Hospital, 20502 Malm?, Sweden;(3) Department of Radiation Biology 6321, Finsen Centre, Rigshospitalet, 2100 Copenhagen ?, Denmark;(4) Institute of Human Genetics, University of Aarhus, Aarhus, Denmark |
| Abstract: | BackgroundPhiC31 integrase facilitates efficient integration of transgenes into human and mouse genomes and is considered for clinical gene therapy. However recent studies have shown that the enzyme can induce various chromosomal abnormalities in primary human embryonic cells and mammalian cell lines. The mechanisms involved are unknown, but it has been proposed that PhiC31 attachment sites in the host genome recombine leading to chromosomal translocations. |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ | |