Streptomycin biosynthesis in Streptomyces griseus II. Adjacent genomic location of biosynthetic genes and one of two streptomycin resistance genes |
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| Authors: | J. Distler K. Mansouri W. Piepersberg |
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| Affiliation: | Institute of Applied Microbiology, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan;Department of Agricultural Chemistry, University of Tokyo, Bunkyo-ku, Tokyo 113, Japan;Sankyo Analytical and Metabolic Research Laboratories, Hiromachi I-chome, Shinagawa-ku, Tokyo 140, Japan |
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| Abstract: | Abstract A new quinone was isolated from the thermophilic methane-oxidizing bacterium strain H-2; was eluted after ubiquinone-8 on reversed-phase high-performance liquid chromatography (HPLC). Proton-magnetic resonance spectroscopy revealed that one of the isoprene units of a side chain was changed to 4-methyl-3-isopentene. The position of the substituted isoprene unit was localized by MS/MS spectrometry. The new quinone was identified as 2,3-dimethoxy-5-methyl-6-geranylgeranyl- [4-methyl-3-isopentenyl]-farnesyl-1,4-benzoquinone. |
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| Keywords: | A new coenzyme Q methyl-substituted isoprenoid MS/MS spectrometry chemotaxonomy |
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