Biosensors for assay of glycoalkaloids in potato tubers |
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| Authors: | V N Arkhypova S V Dzyadevych N Jaffrezic-Renault C Martelet A P Soldatkin |
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| Institution: | (1) Institute of Molecular Biology and Genetics, National Academy of Sciences of Ukraine, Kiev, 03143, Ukraine;(2) Ecole Centrale de Lyon, Ecully Cedex, 69134, France |
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| Abstract: | The possibility of practical application of biosensors based on pH-sensitive field-effect transistors and butyrylcholinesterase to glycoalkaloid analysis in potato tubers was studied. The main analytical features of the designed biosensors and measurement conditions were optimized. The biosensor was applied to quantitative analysis of glycoalkaloids in tubers of different potato varieties. The results proved to be in good agreement with those obtained by conventional protocols. Experiments on glucose assay were performed. An inverse correlation between the contents of glucose and glycoalkaloids in potato tubers was demonstrated. |
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