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| 芸薹链格孢致病毒素分析Ⅰ.腐败菌素B的分离提纯和结构鉴定 | |
| 引用本文： | 马振国,董金皋,樊慕贞,李正平,刘云慧.芸薹链格孢致病毒素分析Ⅰ.腐败菌素B的分离提纯和结构鉴定[J].菌物学报,2000,19(3):354-365. |
| 作者姓名： | 马振国 董金皋 樊慕贞 李正平 刘云慧 |
| 作者单位： | 1. 河北省农科院植物保护研究所 2. 河北农业大学植病系保定 071001 3. 河北农业大学生物技术中心 |
| 基金项目： | 河北省自然科学基金(394134)资助 |
| 摘 要： | 芸薹链格孢（Alternariabrassicae）在PD液体培养基中黑暗培养20d，过滤获得培养滤液，经乙酸乙酯萃取、45℃减压浓缩得AB-粗毒素。AB-粗毒素经HPLC分析，在λ＝206nm波长下具有Ⅰ、Ⅱ、Ⅲ和Ⅳ4个明显的吸收峰。组分Ⅲ的红外吸收光谱、FAB／MASS和1H-NMR与已报道的腐败菌素B基本一致，由此确定组分Ⅲ为腐败菌素B。试验发现，芸薹链格孢的培养滤液、AB-粗毒素和毒素组分Ⅲ均能造成白菜叶片黄化、萎蔫和坏死。 |
| 关 键 词： | 芸薹链格孢 毒素 分离提纯 结构鉴定 |
| 文章编号： | 007-3515(2000)03-0360-0365 |
| 修稿时间： | 1999年7月20日 |
ANALYSIS OF PATHOGENIC TOXIN FROM ALTERNARIA BRASSICAE I.PURIFICATION AND CHEMICAL STRUCTURE IDENTIFICATION OF DESTRUXIN B |
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| Authors: | MA Zhen-Guo DONG Jin-Gao FAN Mu-Zhen LIU Yun-Hui LI Zheng-Ping |
| Abstract: | Alternaria brassicae was inoculated and cultured in PD liquld medium at 20℃ and in darkcondition for 20d. Crude AB-toxin could be obtained by filtration, ethyl acetate extraction and 45℃ evaporation under vacuum. There were Ⅰ,Ⅰ，Ⅲ and Ⅳ four apparent absorption peaks at λ = 206nm by HPLC analysis. Infra-red absorption spectrum， FAB / MASS and 1H-NMR of AB-toxin Ⅲ were idenhcal to those of Destruxin B. The experimental results also showed that culture filtrate of A. brassicae、 crude AB-toxin and punfied AB-toxin Ⅲ all could induced chlorisis, wilt and necrosis symptom on Chinese cabbage leaves. |
| Keywords: | Alternaria brassicae Toxin Isolation and purification Chemical identification |
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