| 商陆抗病毒蛋白基因导入百合愈伤组织初报 |
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| 引用本文： | 王进忠,王彦珺,王锡锋,张民照,高遐虹,周广和.商陆抗病毒蛋白基因导入百合愈伤组织初报[J].生物技术,2005,15(3):73-75. |
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| 作者姓名： | 王进忠 王彦珺 王锡锋 张民照 高遐虹 周广和 |
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| 作者单位： | 1. 北京农学院植物科学技术系,北京,102206
2. 中国农业科学院植物保护研究所,北京,100094 |
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| 基金项目： | 北京市自然科学基金项目资助(编号:5042018，5043026)，北京市农业应用新技术重点实验室资助 |
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| 摘 要： | 利用美洲商陆抗病毒蛋白(Pokeweed antiviral protein，PAP)具有广谱的抗病毒特性，通过冻融法将含有PAP基因的重组表达载体PBll21转入土壤农杆菌LBA4404中，利用叶盘法在农杆菌的介导下转比麝香百合愈伤组织，通过抗性筛选和PCR输测获得了转PAP基因的百合植株。
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| 关 键 词： | PAP基因 麝香百合 转化 检测 |
Transformation of Pokeweed Anfiviral Protein Gene into Calli of Lilium longiflorum |
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| WANG Jin-zhong,WANG Yan-jun,WANG Xin-feng,ZHANG Min-Zhao,GAO Xia-hong,ZHOU Guang-he.Transformation of Pokeweed Anfiviral Protein Gene into Calli of Lilium longiflorum[J].Biotechnology,2005,15(3):73-75. |
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| Authors: | WANG Jin-zhong WANG Yan-jun WANG Xin-feng ZHANG Min-Zhao GAO Xia-hong ZHOU Guang-he |
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| Institution: | WANG Jin-zhong 1,WANG Yan-jun 1,WANG Xin-feng 2,ZHANG Min-zhao 1,GAO Xia-hong 1,ZHOU Guang-he 2 |
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| Abstract: | Calli of Lilium longiflorum leaf were used as receptor of PAP target gene,which was isolated from Pokeweed leaves.Then the PAP gene and Kanamycin resistant gene were cotransformed into the calli by Agrobacterium Tumefaciens .An optimum protocol of transformation has been established and 49 regeneative plants from transformations has been obtained.The PAP cDNA integrated into the calli of lily was assayed by PCR,The results of PCR showed that PAP gene had been transferred and integrated into Lilium longiflorum genomes. |
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| Keywords: | Pokeweed Antiviral Protein(PAP) Lilium longiflorum transformation expression detection |
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