Surface thermodynamics of normal and pathological human granulocytes |
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| Authors: | D. R. Absolom C. J. van Oss R. J. Genco D. W. Francis A. W. Neumann |
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| Affiliation: | (1) Department of Microbiology State, University of New York at Buffalo, 14214 Buffalo, New York;(2) Department of Mechanical Engineering, University of Toronto, M5S 1A4 Toronto, Ontario, Canada;(3) Department of Oral Biology State, University of New York at Buffalo, 14214 Buffalo, New York |
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| Abstract: | Surface tensions of normal and pathological granulocytes were determined by (1) adhesion to solid substrates of different surface tensions while suspended in liquid media of different surface tensions, and by (2) measurement of cell-liquid-vapor contact angles obtained with sessile drops of saline water on cell monolayers. The results obtained by the two different methods were in close conformation with one another. With the cell adhesion emthod some residual leukocyte adhesion still persists even under conditions where there no longer is a van der Waals attraction between cells and solid substrate. At low ionic strength and by the abolishment of all multivalent cations through the admixture of EDTA, that residual cell adhesion virtually disappears (with normal as well as with pathological granulocytes), indicating that the earlier residual cell adhesion did indeed arise from electrostatic interactions mediated by multivalent cations (probably Ca2+). Comparison of the capacities for engulfment and the surface thermodynamics data of normal and pathological granulocytes obtained in this study leads to the novel observation that the phagocytic episode from half to complete engulfment of bacterial particles by granulocytes appears to be the crucial step from the thermodynamic point of view. |
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| Keywords: | Cell adhesion, of normal and pathological granulocytes leukocytes, normal and pathological, cell adhesion in granulocytes, normal and pathological, cell adhesion in cellular surface tension, of normal and pathological granulocytes cell surface thermodynamics, of normal and pathological granulocytes free energy of engulfment, of normal and pathological granulocytes phagocytic activity, of normal and pathological granulocytes |
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