Chick embryo nerve growth factor : Fractionation and biological activity |
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Authors: | Makonnen Belew Ted Ebendal |
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Affiliation: | 1. DSF Department of Pharmaceutical and Pharmacological Sciences, University of Padova, via Marzolo 5, 35121 Padova, Italy;2. DAFNAE Department of Agronomy Animal Foods Natural Resources and Environment, Viale dell''Università, 16, 35020 Legnaro, University of Padova |
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Abstract: | A nerve growth factor (NGF)-like factor initiating nerve fibre outgrowth from sympathetic ganglia in culture was partially purified from chick embryo extract by cation-exchange chromatography followed by hydrophobic interaction chromatography on octylsulfide agarose. The NGF-like factor was markedly activated upon gel filtration in the presence of 6 M urea. Further analysis of the activated chick NGF by immunoblotting following SDS-PAGE, and by inhibition of bioassay response using antibodies to mouse beta NGF demonstrated a distinct antigenic cross-reactivity. The size of the chick embryo NGF was also indistinguishable from that of the mouse beta NGF with a molecular weight (MW) of about 14,000. The findings demonstrate directly the presence of biologically active NGF protein in the developing 18-day chick embryo. |
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