Differential resistance to calcium-induced bilirubin-dependent hemolysis in mammalian erythrocytes |
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| Affiliation: | 1. Department of Animal and Poultry Nutrition, Animal Science Faculty, Gorgan University of Agricultural Sciences and Natural Resources, Gorgan, Iran;2. Faculty of Veterinary Medicine, Islamic Azad University, Babol Branch, Babol, Iran;3. Department of Animal Science, Faculty of Agriculture, University of Tabriz, Tabriz, Iran;1. Department of Pharmacology and Cleveland Center for Membrane and Structural Biology, Case Western Reserve University, Cleveland, OH, United States of America;2. Department of Comparative Medicine, University of Washington, Seattle, WA, United States of America;3. Department of Medicine, College of Physicians and Surgeons, Columbia University, New York, NY, United States of America;1. Programa de Pós-Graduação em Ciências da Nutrição, Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil;2. Departamento de Biologia Molecular, Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil;3. Centro de Desenvolvimento Tecnológico, Universidade Federal de Pelotas, Rio Grande do Sul, Brazil;4. Departamento de Ciências Básicas da Saúde, Universidade Federal de Ciências da Saúde de Porto Alegre, Porto Alegre, Rio Grande do Sul, Brazil;5. Programa de Pós-Graduação em Bioquímica e Bioprospecção, Universidade Federal de Pelotas, Pelotas, Rio Grande do Sul, Brazil;6. Programa de Pós-Graduação em Ciência e Tecnologia dos Alimentos, Universidade Federal da Paraíba, João Pessoa, Paraíba, Brazil;1. Department of Medicine, Laval University, 11, côte du Palais, Québec (Qc), Canada G1R 2J6;2. Cardiometabolic Axis, Kinesiology Department, University of Montréal, 900, rue Saint-Denis, Montréal (Qc), Canada H2X 0A9 |
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| Abstract: | Washed erythrocytes from human, buffalo, sheep and goat preincubated with different concentrations of calcium chloride (16.7–1830 μM) showed significantly different rates of hemolysis (up to 62%) after addition of bilirubin (72 μM). Goat erythrocytes displayed marked resistance to hemolysis with only 11% hemolysis observed at the highest calcium concentration. Similar trend in hemolysis was also observed when the concentration of CaCl2 was fixed (330 μM) and bilirubin concentration varied (0–72 μM). (Ca2+–Mg2+)-ATPase levels were found significantly lower in goat and sheep erythrocyte membranes compared to human and buffalo erythrocyte membranes. This was correlated well with the observed hemolysis in various mammalian erythrocytes. |
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