Bioengineering approaches to mature induced pluripotent stem
cell-derived atrial cardiomyocytes to model atrial fibrillation |
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Authors: | Olivia T Ly Grace E Brown Yong Duk Han Dawood Darbar Salman R Khetani |
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Institution: | 1.Division of Cardiology, Department of Medicine, University of Illinois at Chicago, Chicago, IL 60612, USA; 2.Department of Bioengineering, University of Illinois at Chicago, Chicago, IL 60607, USA; 3.Department of Medicine, Jesse Brown VA Medical Center, Chicago, IL 60612, USA |
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Abstract: | Induced pluripotent stem cells (iPSCs) serve as a robust platform to model several human arrhythmia syndromes including atrial fibrillation (AF). However, the structural, molecular, functional, and electrophysiological parameters of patient-specific iPSC-derived atrial cardiomyocytes (iPSC-aCMs) do not fully recapitulate the mature phenotype of their human adult counterparts. The use of physiologically inspired microenvironmental cues, such as postnatal factors, metabolic conditioning, extracellular matrix (ECM) modulation, electrical and mechanical stimulation, co-culture with non-parenchymal cells, and 3D culture techniques can help mimic natural atrial development and induce a more mature adult phenotype in iPSC-aCMs. Such advances will not only elucidate the underlying pathophysiological mechanisms of AF, but also identify and assess novel mechanism-based therapies towards supporting a more ‘personalized’ (i.e. patient-specific) approach to pharmacologic therapy of AF. |
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Keywords: | Atrial fibrillation human-induced pluripotent stem cell-derived atrial cardiomyocytes disease modeling maturation pharmacologic response |
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