A sensitive method for estimating the carbohydrate content of glycoproteins |
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| Authors: | Gerald Krystal A.F. Graham |
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| Affiliation: | Department of Biochemistry, McGill University, Montreal, Quebec, Canada |
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| Abstract: | A spectrophotometric method is described for estimating the carbohydrate content of acid-hydrolyzed glycoproteins. The method is based on the alkaline ferricyanide reaction with reducing sugars and is accurate in the 1–25 nmole range. Using a simple two-step ion-exchange column procedure both the total sugar content and the proportion of neutral and amino sugars can be determined. |
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