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<Emphasis Type="Italic">Candida rugosa</Emphasis> lipase-catalyzed polyurethane degradation in aqueous medium
Authors:Rajeeb Gautam  Amarjeet S Bassi  Ernest K Yanful
Institution:(1) Department of Chemical and Biochemical Engineering, The University of Western Ontario, London, ON, Canada, N6A 5B9;(2) Department of Civil and Environmental Engineering, The University of Western Ontario, London, ON, Canada, N6A 5B9
Abstract:Candida rugosa lipase (EC 3.1.1.3) was used to degrade commercially-available solid poly(ester)urethane (Impranil) in an aqueous medium under different temperature, pH, enzyme and substrate concentrations. A mathematical model was developed and applied to represent the degradation kinetics of the solid polyurethane. Reaction optima were found to be pH 7 and 35°C. Diethylene glycol, a degradation byproduct, generation rate was measured to be 0.12 mg/l min and the activation energy was calculated as 9.121 kcal/gmol K. This information will be useful in developing bioreactors for practical applications to manage polyurethane wastes using lipase.
Keywords:Candida           rugosa            Lipase  Polyurethane  Biodegradation
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