Mapping of repeated DNA sequences in plant chromosomes by PRINS and C-PRINS |
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| Authors: | M Kubaláková J Macas J Dolez˘el |
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| Institution: | (1) De Montfort University Norman Borlaug Centre for Plant Science Research, Institute of Experimental Botany, Sokolovská 6, CZ 772 00 Olomouc, Czech Republic, XX;(2) Institute of Plant Molecular Biology, Branis˘ovská 31, CZ 370 05 C˘eské Bude˘jovice, Czech Republic, XX |
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| Abstract: | The primed in situ DNA labelling (PRINS) procedure was optimised for the rapid physical mapping of several types of repetitive
DNA sequences on the mitotic chromosomes of Vicia faba, Pisum sativum and Secale cereale. A localization of the highly repeated FokI sequence on V. faba chromosomes was achieved after a 7-min total reaction time. In addition, we report a procedure for direct cycling-PRINS (C-PRINS),
a variation of PRINS which involves a sequence of thermal cycles analogous to the polymerase chain reaction. Compared to PRINS,
C-PRINS was more sensitive. Further work is needed to improve the sensitivity of the reaction to allow for the reliable detection
of low-copy DNA sequences.
Received: 17 September 1996 / Accepted: 18 October 1996 |
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| Keywords: | PRINS C-PRINS Ribosomal RNAgenes Telomeres FokI sequence Repetitive DNA Plant chromosomes |
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