Localization of the human type 5, tartrate-resistant acid phosphatase gene by in situ hybridization |
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Authors: | B S Allen C M Ketcham R M Roberts H S Nick H Ostrer |
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Affiliation: | Department of Biochemistry and Molecular Biology, University of Florida, Gainesville 32610. |
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Abstract: | We report the localization of the gene for the human type 5, tartrate-resistant, iron-containing acid phosphatase isoenzyme (HGM designation ACP5) to chromosome 15 (15q22-q26) using the technique of in situ hybridization to metaphase chromosomes. We have localized this gene using peripheral blood chromosomes obtained from both a normal male and an individual carrying an unbalanced translocation involving chromosome 15 [45,XY,-15,-18,+der(18)-t(15;18)(q13;p11)]. In addition, we have demonstrated the utility of employing a standard fluorescent staining technique (distamycin/DAPI) to an emulsion-coated, Wright-stained, and destained chromosome preparation. |
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