Application of centrifugal force to the extraction and separation of parasorboside and gerberin from Gerbera hybrida |
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Authors: | Yrjönen Teijo Vuorela Pia Klika Karel D Pihlaja Kalevi Teeri Teemu H Vuorela Heikki |
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Institution: | Department of Pharmacy, Division of Pharmacognosy, University of Helsinki, PO Box 56, Helsinki FIN-00014, Finland. |
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Abstract: | A method for the separation of parasorboside and gerberin from the ornamental plant Gerbera hybrida (Asteraceae) has been developed. The two closely related glucosides were extracted using an Extrachrom instrument, a prototype multi-functional separation tool equipped with an extraction chamber. The rotation planar extraction procedure was compared with that of a medium pressure solid-liquid extraction system. The resulting extracts were pre-purified using rotation planar chromatography and the results compared with those obtained using medium pressure liquid chromatography with silica gel as the stationary phase and a mobile phase of methanol:ethyl acetate:tetrahydrofuran at selectivity point Ps = 111 with 1% formic acid as modifier. The title compounds were isolated from the purified extracts by TLC and their structures confirmed by 1H- and 13C-NMR spectroscopy. |
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Keywords: | Extraction methods rotation planar chromatography medium pressure liquid chromatography 2-pyrone derivatives Gerbera hybrida Asteraceae |
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