A Simple Method for Preparing Thin (10 μm) Histological Sections of Undecalcified Plastic Embedded Bone with Implants |
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Authors: | H B M van der Lubbe C P A T Klein K de Groot |
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Institution: |
a Department of Biomaterials Sciences, ACTA, Free University, de Boelelaan, Amsterdam, The Netherlands |
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Abstract: | A simple method for preparing undecalcified thin sections of bone with implants has been developed. After exposing a surface of bone and implant in a plastic block by sawing thick sections, the surface is stained prior to making a thin section. A glass coverslip is affixed with a thin layer of cement to the stained surface to stabilize the tissue and implant during sectioning. A mixture of glycerine and water is used as a coolant and lubricant. The orientation in situ is preserved allowing demonstration of bone architecture and cells, and the tissue-implant interface. |
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