Optimization of polyhydroxybutyrate production by <Emphasis Type="Italic">Bacillus</Emphasis> sp. CFR 256 with corn steep liquor as a nitrogen source |
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Authors: | S V N Vijayendra N K Rastogi T R Shamala P K Anil Kumar L Kshama G J Joshi |
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Institution: | (1) Department of Food Microbiology, Central Food Technological Research Institute, Mysore, 570 020, India;(2) Department of Food Engineering, Central Food Technological Research Institute, Mysore, 570 020, India |
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Abstract: | Polyhydroxyalkanotes (PHAs), the eco-friendly biopolymers produced by many bacteria, are gaining importance in curtailing
the environmental pollution by replacing the non-biodegradable plastics derived from petroleum. The present study was carried
out to economize the polyhydroxybutyrate (PHB) production by optimizing the fermentation medium using corn steep liquor (CSL),
a by-product of starch processing industry, as a cheap nitrogen source, by Bacillus sp. CFR 256. Response surface methodology (RSM) was used to optimize the fermentation medium using the variables such as
corn steep liquor (5–25 g l−1), Na2HPO4 2H2O (2.2–6.2 g l−1), KH2PO4 (0.5–2.5 g l−1), sucrose (5–55 g l−1) and inoculum concentration (1–25 ml l−1). Central composite rotatable design (CCRD) experiments were carried out to study the complex interactions of the variables.
The optimum conditions for maximum PHB production were (g l−1): CSL-25, Na2HPO4 2H2O-2.2, KH2PO4 − 0.5, sucrose − 55 and inoculum − 10 (ml l−1). After 72 h of fermentation, the amount of PHA produced was 8.20 g l−1 (51.20% of dry cell biomass). It is the first report on optimization of fermentation medium using CSL as a nitrogen source,
for PHB production by Bacillus sp. |
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Keywords: | Bacillus Corn steep liquor Central composite rotatable design Optimization Polyhydroxybutyrate Response surface methodology |
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