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Mesenchymal Stem Cells Augment the Anti-Bacterial Activity of Neutrophil Granulocytes
Authors:Sven Brandau  Mark Jakob  Kirsten Bruderek  Friedrich Bootz  Bernd Giebel  Stefan Radtke  Katharina Mauel  Marcus J?ger  Stefanie B Flohé  Stephan Lang
Institution:1. Department of Otorhinolaryngology, University Hospital Essen, Essen, Germany.; 2. Department of Otorhinolaryngology, University Hospital Bonn, Bonn, Germany.; 3. Institute of Transfusion Medicine, University Hospital Essen, Essen, Germany.; 4. Surgical Research, Department of Trauma Surgery, University Hospital Essen, Essen, Germany.; Hannover Medical University (MHH), Germany,
Abstract:

Background

Mesenchymal stem cells (MSCs) participate in the regulation of inflammation and innate immunity, for example by responding to pathogen-derived signals and by regulating the function of innate immune cells. MSCs from the bone-marrow and peripheral tissues share common basic cell-biological functions. However, it is unknown whether these MSCs exhibit different responses to microbial challenge and whether this response subsequently modulates the regulation of inflammatory cells by MSCs.

Methodology/Principal Findings

We isolated MSCs from human bone-marrow (bmMSCs) and human salivary gland (pgMSCs). Expression levels of TLR4 and LPS-responsive molecules were determined by flow cytometry and quantitative PCR. Cytokine release was determined by ELISA. The effect of supernatants from unstimulated and LPS-stimulated MSCs on recruitment, cytokine secretion, bacterial clearance and oxidative burst of polymorphonuclear neutrophil granulocytes (PMN) was tested in vitro. Despite minor quantitative differences, bmMSCs and pgMSCs showed a similar cell biological response to bacterial endotoxin. Both types of MSCs augmented anti-microbial functions of PMNs LPS stimulation, particularly of bmMSCs, further augmented MSC-mediated activation of PMN.

Conclusions/Significance

This study suggests that MSCs may contribute to the resolution of infection and inflammation by promoting the anti-microbial activity of PMNs. This property is exerted by MSCs derived from both the bone-marrow and peripheral glandular tissue.
Keywords:
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