Isolation and Characterization of Atrazine Mineralizing Bacillus subtilis Strain HB-6 |
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| Authors: | Jinhua Wang Lusheng Zhu Qi Wang Jun Wang Hui Xie |
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| Institution: | 1. College of Resources and Environment, Key Laboratory of Agricultural Environment in Universities of Shandong, Shandong Agriculture University, People''s Republic of China.; 2. Ministry of Education Key Laboratory of Pollution Processes and Environmental Criteria, Nankai University, People''s Republic of China.; Loyola University Medical Center, United States of America, |
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| Abstract: | Atrazine is a widely used herbicide with great environmental concern due to its high potential to contaminate soil and waters. An atrazine-degrading bacterial strain HB-6 was isolated from industrial wastewater and the 16S rRNA gene sequencing identified HB-6 as a Bacillus subtilis. PCR assays indicated that HB-6 contained atrazine-degrading genes trzN, atzB and atzC. The strain HB-6 was capable of utilizing atrazine and cyanuric acid as a sole nitrogen source for growth and even cleaved the s-triazine ring and mineralized atrazine. The strain demonstrated a very high efficiency of atrazine biodegradation with a broad optimum pH and temperature ranges and could be enhanced by cooperating with other bacteria, suggesting its huge potential for remediation of atrazine-contaminated sites. To our knowledge, there are few Bacillus subtilis strains reported that can mineralize atrazine, therefore, the present work might provide some new insights on atrazine remediation. |
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