High-Throughput Sequencing of Plasma MicroRNA in Chronic Fatigue Syndrome/Myalgic Encephalomyelitis |
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| Authors: | Ekua W Brenu Kevin J Ashton Jana Batovska Donald R Staines Sonya M Marshall-Gradisnik |
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| Institution: | 1. School of Medical Science, Griffith Health Centre, Griffith University, Gold Coast, Queensland, Australia.; 2. The National Centre for Neuroimmunology and Emerging Diseases, Griffith University, Gold Coast, Queensland, Australia.; 3. Faculty of Health Sciences and Medicine, Bond University, Robina, Queensland, Australia.; 4. Queensland Health, Gold Coast Public Health Unit, Robina, Gold Coast, Queensland, Australia.; St. Georges University of London, United Kingdom, |
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| Abstract: | BackgroundMicroRNAs (miRNAs) are known to regulate many biological processes and their dysregulation has been associated with a variety of diseases including Chronic Fatigue Syndrome/Myalgic Encephalomyelitis (CFS/ME). The recent discovery of stable and reproducible miRNA in plasma has raised the possibility that circulating miRNAs may serve as novel diagnostic markers. The objective of this study was to determine the role of plasma miRNA in CFS/ME.ResultsUsing Illumina high-throughput sequencing we identified 19 miRNAs that were differentially expressed in the plasma of CFS/ME patients in comparison to non-fatigued controls. Following RT-qPCR analysis, we were able to confirm the significant up-regulation of three miRNAs (hsa-miR-127-3p, hsa-miR-142-5p and hsa-miR-143-3p) in the CFS/ME patients.ConclusionOur study is the first to identify circulating miRNAs from CFS/ME patients and also to confirm three differentially expressed circulating miRNAs in CFS/ME patients, providing a basis for further study to find useful CFS/ME biomarkers. |
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