HCMV大小鼠眼组织损伤模型的初步研究

目的探讨接种人巨细胞病毒(Humancytomegalovirus,HCMV)是否引起Wistar大鼠、昆明种小鼠眼组织损伤.方法将HCMVAD169毒株经静脉接种Wistar大鼠和昆明种小鼠,观察动物眼部发病情况,原位杂交检测动物眼组织中的HCMVDNA片段.结果接种病毒后部分动物缓慢出现眼部发病,局部分泌物增多、浑浊甚至失明;经原位杂交于视锥、视杆细胞和角膜内皮细胞中检出HCMVDNA片段.结论HCMV可以感染动物眼组织,并引起动物发生眼病.

Study on Eye Disease of Animal Infected with HCMV AD169 Strain

Objective To investigate whether HCMV infection can cause ocular tissue lesions in rats and mice.Methods Twenty rats and ten mice were inoculated with HCMV AD 169 strain by vein and development of ocular disease was observed.HCMV DNA of tissues from inoculated animals were examined by in situ hybridization.Results Some animals expressed much secretion,turbidity in eyes even losing sight at different time post|inoculation.HCMV DNA sections were identified in the corneal endothelium cell,rod cell and cone cell of the rats and mice inoculated. Conclusion HCMV AD 169 strain can infect rats and mice and cause ocular disease.;