Regions associated with the stable maintenance of plasmid pSC101 and its tetracycline resistance |
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| Authors: | Souichi Makino Chihiro Sasakawa Hirofumi Dambara and Masanosuke Yoshikawa |
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| Institution: | (1) Department of Bacteriology, Institute of Medical Science, University of Tokyo, 108 Minato-ku, Tokyo, Japan |
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| Abstract: | Summary Two regions tentatively called unsA and unsR were identified on pSC101. One, unsA, corresponds to less than 650 bp of the N-terminal in the tetracycline resistance structural gene and seems to inhibit stable maintenance of pSC101. The other, unsR, is defined within the 1 kb XhoI-EcoRI region located upstream of the tetracycline resistance structural gene and is a regulatory gene clearly distinct from tetR (Unger et al. 1984); it serves as a suppressor of the unsA function. |
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| Keywords: | pSC101 Tetracycline Stability Repressor Bal31 |
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