Successful cardiac allografts in syngeneic radiation chimeras

Summary Successful cardiac allografts were accomplished across the major histocompatibility complex of rats. LEW and F344 (Ag-B ²) rats were lethally irradiated and grafted with WF (Ag-B ¹) hearts on day 0. Either on day 0 or day 2, the hosts were repopulated with syngeneic hemopoietic cells. The best results were obtained (86%) when a mixture of 3.0 × 10⁷ non-adherent syngeneic bone marrow and thymus cells were used to repopulate the recipients. In contrast, all of the WF to LEW heart grafts were rejected within 30 days if syngeneic thoracic duct and bone marrow cells were used to repopulate the host.Supported by Grant HL18186 from the National Institutes of HealthRecipient of a Research Career Development Award CA70879 from the National Institutes of Health