Detection and determination of selenoproteins by nuclear techniques |
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Authors: | Stone S F Bernasconi G Haselberger N Makarewicz M Ogris R Wobrauschek P Zesler R |
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Institution: | 1.Division of Human Health and Agenyy's Laboratories Seibersdorf, International Atomic Energy Agency, POB 100, A-1400, Vienna, Austria ;2.Atominstitut der ?sterreichischen Universit?ten, Schüttelstrasse 115, A-1020, Vienna, Austria ; |
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Abstract: | Microbeam X-ray spectrometry, energy-dispersive X-ray fluorescence analysis, and neutron activation analysis were evaluated
for the detection of selenium contained in the selenoprotein glutathione peroxidase. The glutathione peroxidase had been previously
separated using polyacrylamide gel electrophoresis. The use of Bragg-reflected polarized X-ray beams was employed in the X-ray
fluorescence measurements to minimize the problem of scatter owing to the gel matrix. Current detection limits of selenium
in a gel matrix are 2.1 ng in the bench-top microbeam X-ray system and 30–60 ng using XRF with polarized beams. Neutron activation
analysis was used for qualitycontrol measurements, with a detection limit here of <0.08 ng. The work has in principle established
the feasibility of such an approach. |
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Keywords: | Index Entries" target="_blank">Index Entries Polyacrylamide gel electrophoresis (PAGE) protein metalloprotein selenoproteins energy-dispersive X-ray fluorescence (EDXRF) microbeam X-ray spectrometry instrumental neutron activation analysis (INAA) |
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