Determination of effective diffusivity of substrate in biological films and particles |
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Authors: | S. M. Rao Bhamidimarri P. F. Greenfield |
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Affiliation: | (1) Department of Biotechnology, Massey University, Palmerston North, New Zealand;(2) Department of Chemical Engineering, University of Queensland, St. Lucia, Queensland, Australia |
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Abstract: | Summary Particle supported biofilms of uniform thickness were generated in an aerobic fluidized-bed reactor with phenol as the carbon source. A method was developed for determining the effective diffusivities of oxygen and phenol using trypan blue, a vital stain as the tracer. The effective diffusivities of oxygen and phenol were found to be 2.72×10–6 cm2/s and 1.12×10–6 cm2/s respectively.Nomenclature Ci initial solute concentration in bulk, g/cm3 - Ct solute concentration in bulk at time t, g/cm3 - C bulk solute concentration at equilibrium, g/cm3 - D molecular diffusivity, cm2/s - D effective diffusivity, cm2/s - Do Dp Dtb molecular diffusivity of oxygen, phenol and trypan blue, cm2/s - Do, Dp, Dtb effective diffusivity of oxygen, phenol and trypan blue, cm2/s - Ds molecular diffusivity of substrate, cm2/s - Ds effective diffusivity of substrate, cm2/s - K partition coefficient - Mt amount of solute in the particle at time t, g - M amount of solute in the particle at equilibrium, g - r particle radius, cm - rbp radius of the particle with biofilm, cm - S substrate concentration, g/cm3 - Sb substrate concentration in bulk, g/cm3 - Si initial substrate concentration, g/cm3 - V1 solute molar volume, cm3/g molGreek Symbols bf porosity of the biofilm - tortuosity factor |
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