Overproduction of highly active trypanosome alternative oxidase in Escherichia coli heme-deficient mutant |
| |
| Authors: | Fukai Yoshihisa Nihei Coichi Kawai Keisuke Yabu Yoshisada Suzuki Takasi Ohta Nobuo Minagawa Nobuko Nagai Kazuo Kita Kiyoshi |
| |
| Institution: | Department of Biomedical Chemistry, Graduate School of Medicine, University of Tokyo 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. |
| |
| Abstract: | Cyanide-insensitive trypanosome alternative oxidase (TAO) is the terminal oxidase of the respiratory chain of long slender bloodstream forms of the African trypanosome, which causes sleeping sickness in humans and nagana in cattle. TAO has been targeted for the development of anti-trypanosomal drugs, because it does not exist in the host. In this study, we established a system for overproduction of highly active TAO in Eschericia coli heme-deficient mutant. Kinetic analysis of recombinant enzyme and TAO in Trypanosoma brucei brucei mitochondria revealed that recombinant TAO retains the properties of native enzyme, indicating that recombinant TAO is quite valuable for further biochemical study of TAO. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
