Improvement of the production of 5-methoxypodophyllotoxin using a new selected root culture of Linum flavum L. |
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Authors: | Wim van Uden Niesko Pras Ben Homan Theo M Malingré |
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Institution: | (1) Department of Horticulture, The Ohio State University, Ohio Agricultural Research and Development Center, 43210 Columbus, OH, USA |
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Abstract: | Differences in transformation of the tomato cultivar (Ohio 7870, Roma, UCD82b) by wild-type Agrobacterium strains (A6, A66, A281) were identified in a leaf disk assay system. Transformation was expressed as the percentage of explants producing callus on hormone-free medium and was confirmed by opine production. Ohio 7870 and Roma were more readily transformed than UCD82b by all three strains of A. tumefaciens. Cotyledons and older true leaves of all three cultivars were more readily transformed than younger leaves. Transformation was biphasic over the bacterial concentrations tested (2×103–7×109 colony forming units ml-1; cfu ml-1) for all cultivars and leaf ages, and was greatest at 5×108 cfu ml-1. Transformation decreased significantly at levels less than 2×107 cfu ml-1 and slightly at concentrations higher than 5×108 cfu ml-1. UCD82b tissue was more necrotic than Ohio 7870 or Roma after incubation with bacteria, which may account for reduced transformation of this cultivar. |
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Keywords: | crown gall leaf disk assay Lycopersicon esculentum tomato |
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