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Capturing B type acute lymphoblastic leukemia cells using two types of antibodies |
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| Authors: | Kutay İçöz Tayyibe Gerçek Ayşegül Murat Servet Özcan Ekrem Ünal |
| Affiliation: | 1. BioMINDS (Bio Micro/Nano Devices and Sensors) Lab, Dept. of Electrical and Electronics Engineering, Abdullah Gül University, Kayseri, Turkey;2. BioMINDS (Bio Micro/Nano Devices and Sensors) Lab, Dept. of Electrical and Electronics Engineering, Abdullah Gül University, Kayseri, Turkey Bioengineering Dept., Abdullah Gül University, Kayseri, Turkey;3. Genome and Stem Cell Center (GENKOK), Erciyes University, Kayseri, Turkey;4. Biology Dept., Erciyes University, Kayseri, Turkey Genome and Stem Cell Center (GENKOK), Erciyes University, Kayseri, Turkey;5. Pediatric Oncology Dept., Erciyes University, Kayseri, Turkey |
| Abstract: | One way to monitor minimal residual disease (MRD) is to screen cells for multiple surface markers using flow cytometry. In order to develop an alternative microfluidic based method, isolation of B type acute lymphoblastic cells using two types of antibodies should be investigated. The immunomagnetic beads coated with various antibodies are used to capture the B type acute lymphoblastic cells. Single beads, two types of beads and surface immobilized antibody were used to measure the capture efficiency. Both micro and nanosize immunomagnetic beads can be used to capture B type acute lymphoblastic cells with a minimum efficiency of 94% and maximum efficiency of 98%. Development of a microfluidic based biochip incorporating immunomagnetic beads and surface immobilized antibodies for monitoring MRD can be an alternative to current cost and time inefficient laboratory methods. © 2018 American Institute of Chemical Engineers Biotechnol. Prog., 35: e2737, 2019 |
| Keywords: | B type acute lymphoblastic leukemia cells cell capture efficiency minimal residual disease immunomagnetic separation two antibody sorting |